Catalogue Number: AB01391-41.0-BS-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Monoclonal Secondary Antibody - Unconjugated |
| Alias: | immunoglobulin E; canine IgE; canine immunoglobulin E; D9 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Dog |
| Clone: | D9#3116 |
| Isotype: | IgG1 |
| Immunogen: | This antibody was raised by immunising BALB/c mice with IgE-rich pooled parasitised dog serum and subsequent creation of hybridomas. |
| Application: | ELISA, WB |
Purified
Recombinant Monoclonal
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
ELISA: Canine IgE-specificity of the D9#3116 antibody was confirmed by ELISA tests with different types of antibodies (DeBoer et al., 1993). WB: This antibody was successfully used in western blot detection of canine IgE in experiments on the dogs' allergies against the hen egg (Shimakura et al., 2016). ELISA: fluorometric ELISA tests were used to assay canine IgE reactivity against different vaccine components in anti-vaccine allergies (Ohmori et al., 2005).
This chimeric dog antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.