Anti-GABAR [1F4]

Catalogue Number: AB01411-3.0-BT-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Shipping Condition:Blue Ice
Unit(s): 1 mg
Host name: Mouse
Clone: 1F4
Isotype: IgG2b
Immunogen: This antibody was raised against α1β2γ2 GABA-A receptor.
Application: Crstapy, FSEC

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Gene ID

2554; 2561; 2566

Gene Name

GABRA1, GABRB2, GABRG2

Specificity

Gamma-aminobutyric acid receptor A; Gamma-aminobutyric acid receptor; Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit gamma-2; GABA(A); GABA-A receptor; α1β2γ2 GABA-A receptor

Uniprot ID

P14867, P18507, P47870

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

FSEC: High affinity and specificity of the 1F4 antibody to α1β2γ2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the α1β2γ2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the α1β2γ2 GABA-A receptor (Zhu et al., 2018).