Anti-metarhodopsin-I [K42-41L]

Catalogue Number: AB01414-1.1-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:opsin-2; meta I rhodopsin; rhodopsin; opsin 2; metarhodopsin I
Shipping Condition:Blue Ice
Unit(s): 200 ug
Host name: Mouse
Clone: K42-41L
Isotype: IgG1
Immunogen: This antibody was raised by immunising mice with rhodopsin.
Application: ELISA, ICC, EM

Additional Text

Gene ID

6010

Uniprot ID

P08100

Gene Name

RHO

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

Antibody imprint: This antibody was used to investigate the structure of rhodopsin's meta-states; the researchers employed the antibody imprint technique to analyse conformational changes which this molecule undergoes upon activation by light (Bailey et al., 2003). Another group used K42-41L antibody to demonstrate that it is capable of discriminating between metarhodopsins I and II, and that it stabilises metarhodopsin I while inhibiting metarhodopsin II; these data suggest that this antibody might be useful in the structural studies of opsins (Piscitelli et al., 2006). EM ICC: K42-41L antibody was utilised to stain different structures of Xenopus laevis retinas in EM immunocytochemistry LR white samples in a study investigating opsin localisation (Besharse and Wetzel, 1995).