Anti-PD-1 [J43]
Catalogue Number: AB01417-2.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD279; PD1; PD 1; Programmed cell death protein 1; Programmed cell death 1; pdcd1; Protein PD-1; mPD-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | J43 |
| Isotype: | IgG2a |
| Immunogen: | This antibody was raised by immunising Armenian hamsters with B12 cells, a PD-1 cDNA transfectant of BHK cells. |
| Application: | ELISA, FC, IP, IHC, NT, Depl |
Additional Text
Gene Name
PDCD1
Uniprot ID
Q02242
Gene ID
18566
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The specificity of this antibody has been confirmed in ELISA analysis, using PD-1 extracellular domain fusion proteins (Agata et al, 1996). Additionally, in flow cytometric analysis, this antibody reacts with PD-1 cDNA-transfected BHK and CHO cells, but not with parental BHK and CHO cells, as well as reacting with lymphocytes from PD-1 cDNA transgenic mice (Agata et al, 1996). This antibody has been used to immunoprecipitate PD-1 from lysates of PD-1 cDNA-transfected BHK and CHO cells (Agata et al, 1996), in flow cytometric quantification of CD4+PD-1+ T cells in murine spleens (Kasagi et al, 2010), and in immunohistochemical analysis of acetone-fixed murine spinal cord and brain tissue sections (Salama et al, 2003). This antibody displays diverse effects in different mouse models of disease. When administered to NZB/W F1 mice, a model of lupus-like nephritis, this antibody has been shown to delay the onset of nephritis and prolong survival, through the depletion of PD-1+ T cells (Kasagi et al, 2010). Antibody-treated NZB/W F1 mice displayed decreased numbers of PD-1+ T cells, and this antibody was confirmed to trigger complement-dependent cytotoxicity in PD-1+ T cells in vitro (Kasagi et al, 2010). Conversely, administration to experimental allergic encephalitis (EAE) and NOD diabetes mice exacerbated disease, through its neutralising activity (Salama et al, 2003; Ansari et al, 2003); this antibody has been shown in vitro to inhibit binding of both PD-L1-Ig and PD-L2-Ig to PD-1 transfected BHK cells (Ansari et al, 2003).
Short Description
This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
AB01417-2.3 Datasheet
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