Anti-Fc [TP897]
Catalogue Number: AB01432-34.11-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | fragment crystallizable region; Fc region |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 ug |
| Host name: | Camelid |
| Clone: | TP897 |
| Isotype: | |
| Immunogen: | This antibody was raised by immunising alpacas with 1.0 mg polyclonal rabbit IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed. |
| Application: | ICC, IF, IP, WB, DB |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
Anti-IgG nanobodies can often perform more efficiently than polyclonal secondary antibodies in various experimental assays. For instance, this nanobody conjugated with a maleimide-activated HRP was used to detect rabbit IgG via ECL Western blot and it considerably outperformed a polyclonal secondary antibody-HRP conjugate (Pleiner et al., 2018). Similarly, when conjugated with the infrared fluorophore IRDye 800, it was very efficient in detecting nucleoporins (Nups) in a Xenopus laevis egg extract via Western blot (Pleiner et al., 2018). TP897 antibody can be used for the purification of antigens or antigen complexes previously bound to a primary antibody, as it successfully isolated polyclonal rabbit IgG from serum and then released it under physiological conditions using SUMOStar protease cleavage (Pleiner et al., 2018). This nanobody is also recommended for the immunofluorescence detection of both monoclonal and polyclonal primary rabbit antibodies (Pleiner et al., 2018).
AB01432-34.11-BT Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations