Anti-Fab (kappa) [TP1170]

Catalogue Number: AB01433-34.11-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	antigen-binding fragment kappa light chain
Shipping Condition:	Blue Ice
Unit(s):	50 ug
Host name:	Camelid
Clone:	TP1170
Isotype:
Immunogen:	This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
Application:	ICC, IF, WB, DB

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

Anti-IgG nanobodies can often perform more efficiently than polyclonal secondary antibodies in various experimental assays. For instance, this nanobody when conjugated with the infrared fluorophore IRDye 800 was more efficient in terms of the detection sensitivity of IgG2a antibodies than the polyclonal secondary antibody via Western blot (Pleiner et al., 2018). TP1170 can also be used as an efficient secondary nanobody in the indirect immunofluorescence. It was used in combination with other nanobodies to detect both mouse IgG1 and IgG2a primary antibodies giving strong, clean signal without any background staining (Pleiner et al., 2018). This nanobody is also highly recommended for superresolution microscopy, as it can place the fluorophore much closer to the antigen which had been bound by the primary antibody than a usual secondary antibody would be able to (Pleiner et al., 2018).

AB01433-34.11 Datasheet

Price

£525.00

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