Anti-IgG1/2a Fab (k) [TP888]

Catalogue Number: AB01449-34.11-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Monoclonal Secondary Antibody - Unconjugated
Alias:	immunoglobulin G1; immunoglobulin G2a; antigen binding fragment; kappa light chain
Shipping Condition:	Blue Ice
Unit(s):	50 ug
Host name:	Camelid
Clone:	TP888
Isotype:
Immunogen:	This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
Application:	DB

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

Anti-IgG nanobodies often perform more efficiently than polyclonal secondary antibodies in various experimental assays. Therefore, they are recommended for various experimental procedures. Anti-IgG nanobodies were shown to be more sensitive during Western blots when conjugated with different molecules (HRP, fluorophores etc.) (Pleiner et al., 2018). Furthermore, combination of two distinct nanobodies for immunofluorescence labelling provided exceptionally clean and strong signal, unprecedented among monovalent secondary antibodies (Pleiner et al., 2018). Additionally, using such an antibody allows skipping two-step incubation procedures during immunostaing as the nanobody can be first incubated with a primary antibody and this conjugate might be added to the cells in a one-step procedure (Pleiner et al., 2018). Finally, such nanobodies have been recommended as very effective for colocalisation studies when more than one fluorophore is needed (Pleiner et al., 2018).

AB01449-34.11 Datasheet

Price

£525.00

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