Anti-i-motif DNA [iMab]

Catalogue Number: AB01462-10.0-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	intercalated motif; i-motif; intercalated DNA motif; I motif; DNA i-motif; human telomere i-motif ; hTelo i-motif; imotif; imotiv; imotive; i-motiv; i-motive
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Human
Clone:	iMab
Isotype:	IgG1
Immunogen:	The original antibody was selected from the Garvan-2 human scFv library via phage display.
Application:	ELISA, IF, CM, Mcrspy

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Short Description

This chimeric human IgG1 antibody was made using the variable domain sequences of the original scFv format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The original human scFv version of this antibody was validated for its specificity towards various i-motif types through ELISA and bio-layer interferometry (BLI), with a dissociation constant (KD) of 59 nM. It selectively binds to i-motifs, including those in 1G22, 1A83, c-MYC, RET, and VEGF sequences, located in regions such as telomeres, centromeres, and proto-oncogene promoters. Importantly, this antibody does not interact with structures resembling i-motif DNA, such as DNA G4 quadruplex forms, double-stranded DNA (dsDNA), hairpin DNA, miRNA, or mutant human telomeres lacking i-motifs (Zeraati et al., 2018; PMID: 29686376). Notably, it does not bind to various DNA G4 quadruplex forms, including promoter G4s c-MYC44, BCL245, VEGF, telomeric 2JSM47, 2GKU, or aptamer G4 TBA. Additionally, the antibody has proven effective for immunofluorescence detection of i-motifs in multiple cell lines (MCF7, U2OS, and HeLa), confirming the presence of i-motif structures in human nuclei and their dependence on pH and cell-cycle stage (Zeraati et al., 2018; PMID: 29686376). This antibody selectively binds to both intramolecular and intermolecular i-motif structures. The composition of buffers used during binding and washing steps significantly influences the antibody's selectivity, emphasizing the importance of optimizing DNA concentrations, blocking conditions, and washing protocols. These factors are crucial for confirming the antibody's selectivity for i-motifs and ensuring accurate detection of both intramolecular and intermolecular i-motifs (Ruggiero et al., 2024; DOI: 10.1101/2024.06.22.600195v1).

AB01462-10.0-BT Datasheet

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£1,655.00

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