Anti-CD115/M-CSFR [AFS98]

Catalogue Number: AB01491-2.0-VXM-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:CSF-1R; Macrophage colony-stimulating factor 1 receptor; Csf1r; CD115; CSF-1 receptor; EC 2.7.10.1; CSF-1-R; M-CSF-R; Proto-oncogene c-Fms
Shipping Condition:Blue Ice
Unit(s): 5 mg
Host name: Mouse
Clone: AFS98
Isotype: IgG2a
Immunogen: This antibody was raised against CD115.
Application: FC, WB, IHC, Blk

Additional Text

Gene Name

CSF1R

Gene ID

12978

Uniprot ID

P09581

Antibody Clonality

Recombinant Monoclonal

Storage Note

All VivopureX products are formulated in PBS only without addition of preservatives. To ensure optimal storage and prevent microbial contamination, only open and dispense under sterile conditions. Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

AFS98 has been widely used in various flow cytometric analyses to label CD115 antigen. For instance, one group used it to detect monocytes in a mouse blood sample (Buschor et al., 2019). Similarly, Huo and colleagues (2017) utilised this antibody to identify monocytes in mouse blood in order to assess whether myelopoiesis was not a cause of the increase in the monocytes number. This antibody has been also successfully used to deplete macrophages (Ho et al., 2016). Finally, it was also utilised in the immunofluorescence staining of tissue sections prepared from 4% paraformaldehyde-fixed and decalcified bones (Dong et al., 2016).

Short Description

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.