Anti-SIRP alpha [SAF10.1]
Catalogue Number: AB01531-8.1-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rat |
| Clone: | SAF10.1 |
| Isotype: | IgG2b |
| Immunogen: | This antibody was raised by immunisisng BALB/c mice with an eukaryotically expressed SIRPα-Fc fusion protein consisting of the extracellular domain and emulsified with complete Freund's adjuvant and followed by two subsequent boosts of SIRPα-Fc fusion protein emulsified with IFA (Lee et al., 2007). |
| Application: | FC, IF, IP, WB, BndA |
Additional Text
Gene ID
140885
Gene Name
SIRPA
Uniprot ID
P78324
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity
Tyrosine-protein phosphatase non-receptor type substrate 1; SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha-1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84; CD172a
Application Notes
SAF10.1, can be used to detect SIRPs in a wide variety of tissues and cell types. For instance, this antibody was successfully used to detect SIRPs from COS7.2 cell lysates via Western blot (Lee et al., 2008).
Short Description
This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB01531-8.1 Datasheet
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