Anti-CD40 [2C5]
Catalogue Number: AB01617-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD40L receptor; CDw40; Bp50; B-cell surface antigen CD40; Tumor necrosis factor receptor superfamily member 5 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | 2C5 |
| Isotype: | IgG |
| Immunogen: | This antibody was raised by immunising mice with a recombinant protein corresponding to the extracellular domain of chicken CD40 (chCD40ED). |
| Application: | FC, IF, Agon |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
2C5 antibody is a CD40 agonist and can be used to induce immune activation. Agonistic monoclonal anti-CD40 antibodies (mAbs) mimic the effects of CD40L on APCs, leading to enhanced T-cell priming andexpansion, increased antibody production and class switching. This antibody was shown to be a powerful adjuvant eliciting a strong IgG-response in chicken what makes it an important agent in vaccines development (Chen et al., 2012). Furthermore, 2C5 was also demonstrated to be effective in detecting CD40 via immunofluorescence as well as via flow cytometry in chicken B cells and macrophages (Chen et al., 2010). Finally, this antibody was observed to activate NO synthesis in HD11 macrophages, and stimulate DT40 B-cellproliferation (Chent et al., 2010).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB01617-23.0-BT Datasheet
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