Anti-Cytotoxin 1 [TPL0027_01_A2]

Catalogue Number: AB01641-32.1-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	CTX1; CX1; Cardiotoxin F8; Cardiotoxin-I; CTX-I; Toxin CM-6; toxin CX1
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Sheep
Clone:	TPL0027_01_A2
Isotype:	IgG
Immunogen:	The antibody was raised using IONTAS phage display library, a naive human scFv library consisting of 4.0 10^10 unique antibody fragments. Through phage display selection, scFv-presenting phages were allowed to bind a toxin, phages with unspecific binding were washed away, and the rest were eluted through enzymatic digestion. These were then amplified and used for the following round. In total three rounds of selections were completed leaving the best binders behind.
Application:	ELISA

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

TPL0027_01_A2 antibody has a very high affinity to the cytotoxin 1. It was demonstrated by normalized ELISA, which circumvents the limitation of a simple ELISA by removing the expression variable, normalizing the amount of scFv in each well. The antibody's high affinity was confirmed by its ability to bind the target protein in a venom fraction even at 1:32,000 dilution (3.125 10^-2) µg/mL (Juergensen et al., 2019 [poster]). It is recommended for the research on the antitoxin against Naja nigricollis snakebites.

Short Description

This full-length, chimeric sheep antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

AB01641-32.1 Datasheet

Price

£390.00

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