Anti-beta catenin [15B8]

Catalogue Number: AB01655-23.0-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:Catenin beta-1; Beta-catenin; β-catenin; β catenin
Shipping Condition:Blue Ice
Unit(s): 200 ug
Host name: Rabbit
Clone: 15B8
Isotype: IgG
Immunogen: This antibody was raised by immunizing mice with a recombinant full length β-catenin of chicken origin.
Application: FC, IF, WB, IHC, EMSA

Additional Text

Gene Name

CTNNB1

Gene ID

1499

Uniprot ID

P35222

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody is recommended for the detection and analysis of beta-catenin via various methods, such as Western blot, flow cytometry and immunofluorescence of cells and tissues. For instance, the antibody 15B8 was utilized in labelling beta-catenin in oral mucosa samples via immunohistochemistry (Das et al., 2013). Another group used this antibody in the identification of beta-catenin in extracts from K562 cells via Western blots (Kotula et al., 2014). Furthermore, this antibody was applied during immunohistochemistry stainings of transverse sections taken through the midbrain region of 8-9ss embryos (Schiffmacher et al., 2016). Finally,beta-catenin is a very popular marker used to outline the cell membrane, this antibody might be utilized for such purposes (Zhang et al., 2017).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.