Anti-CD74 [LL1 (EPB1)]

Catalogue Number: AB01684-3.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Ii; p33; DHLAG; HLADG; Ia-GAMMAHLA; HLA Class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Mouse
Clone:	LL1 (EPB1)
Isotype:	IgG2b
Immunogen:	This antibody was generated by immunizing female BALB/c mice with whole Raji cells or a crude membrane preparation.
Application:	FC, IP, WB, RIA

Overview
Datasheets

Additional Text

Gene ID

972

Gene Name

CD74

Uniprot ID

P04233

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

Radioimmunobinding and flow cytometric analysis was used to analyse binding of LL1 to peripheral blood. LL1 did not react with T cell lines nor with normal diploid cells. The reactivity of LL1 (EB-1) to the tumor cells was checked using a live cell indirect immunoflurescence staining assay. LL1 stained positive for cell lines Raji (maxilla), Daudi (B-lymphoblast), RAMOS (b-lymphocyte), SU-DHL-7, ARH-77 (plasma cell leukemia), HS-Sultan (plasmacytoma). LL1 is reported to recognize most of the small and large lymphocytes of the germinal centre and no reactivity to marginal zone B-cells. It is also reported to react with interdigitating histiocytes of the T-cell zones. It was further reported that LL1 bound positively to Western blotted Raji cell antigens (PMID: 2663143). The specificity of LL1 for Ii subunit was determined by immunoprecipitation. Radiolabelled antibodies were used for internalization and catabolism of the antibody and it was seen that LL1 was internalized and catabolized very quickly as compared to some other antibodies like those which are specific for CD19, CD22 and anti-transferrin receptor. (PMID: 8947500). LL1 was evaluated to be a potential candidate for bone marrow imaging using Tcm labelled LL1 sulphur colloid imaging. (PMID: 9076770)

Short Description

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB01684-3.0 Datasheet

Price

£390.00

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