Anti-4-1BB [4B4-1-1]
Catalogue Number: AB01689-3.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD137; MGC2172; Tumor necrosis factor receptor superfamily member 9 (TNFRSF9); T cell antigen ILA; Interleukin activated receptor; 4-1BB ligand receptor CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA. |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | 4B4-1-1 |
| Isotype: | IgG2b |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with a fusion protein of human 4-1BB antigen and glutathione s transferase (GST). |
| Application: | ELISA, FC, IHC, Blk |
Additional Text
Gene ID
3604
Gene Name
TNFRSF9
Uniprot ID
Q07011
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The antibody has good binding affinity for Con A-activated T cells and has an immunosuppressive activity towards lymphocyte proliferation in the in vitro mixed lymphocyte reaction. The binding of antibody to surface expressed 4-1BB molecule was assessed using flow cytometry and ELISA was used to check whether the cell culture had binding affinity to 4-1BB (Kim et al., 1995). The interaction of this antibody with CD137 is reported to induce an increase in TNF-α production by monocytes. This antibody competes with mAb M121 for binding to CD137 (PMID: 10607752). This antiboy also induces the production of IFN-γ in NK cells in presence of IL-15 (PMID: 20008791).This antibody can be used in blocking assays and staining methods (PMID: 14746806). Humanized versions of this antibody (Hz4B4-1) and (Hz4B4-2) showed a 1.5 fold and 7.3 fold increase and in binding affinity for 4-1BB as compared to their parental mouse antibody. Additionally, H4B4 mediated antibody-dependent cellular toxicity of activated human peripheral blood T cells and CEM cells in a dose dependent manner (PMID: 11186149). Humanized version of this antibody is available on request.
Short Description
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB01689-3.0 Datasheet
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