Anti-PERV [A5]
Catalogue Number: AB02000-10.159-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Porcine Endogenous Retrovirus; gag protein; p15 matrix protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Human |
| Clone: | A5 |
| Isotype: | IgG1 |
| Immunogen: | This variable heavy chain fragment was prepared by immunization of a young adult male Llama glama with a purified 60-kDa PERV-B gag protein. The gag protein was prepared by amplifying PERV-B gag cDNA from PK15 cell RNA using a forward and reverse primer and later on cloning it into the pET-30a expression vector. |
| Application: | WB |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This variable single heavy chain antibody to 60kDa gag polyprotein and p15 matrix protein of the PERV and its binding was confirmed by Western blotting. The antibody has shown to reduce the (PERV-A/B) virus production when it is intracellularly expressed in PK cells. This antibody with its high affinity for p15 matrix protein of the PERV gag polyprotein, solubility in aqueous environments and intracellular expression shows potential to be used as an intrabody to block the production of PERV and prevent risk of infections or transmission of infectious diseases (xenozoonosis) when tissues and organs from genetically modified pigs are to be used in xenotransplantation (Dekker et al., 2003).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
AB02000-10.159 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations