Catalogue Number: AB02001-10.159-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Porcine Endogenous Retrovirus; gag protein; p27 capsid protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 ug |
| Host name: | Human |
| Clone: | D2 |
| Isotype: | IgG1 |
| Immunogen: | This variable heavy chain fragment was prepared by immunization of a young adult male Llama glama with a purified 60-kDa PERV-B gag protein. The gag protein was prepared by amplifying PERV-B gag cDNA from PK15 cell RNA using a forward and reverse primer and later on cloning it into the pET-30a expression vector. |
| Application: | WB |
Purified
Recombinant Monoclonal
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
This variable single heavy chain antibody to 60kDa gag polyprotein and p27 major capsid protein of the PERV and its binding was confirmed by Western blotting. This antibody could not reduce the (PERV-A/B) virus protein production when it is intracellularly expressed in PK cells (Dekker et al., 2003).
This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.