Anti-HCMV [8F9]
Catalogue Number: AB02002-10.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | gB; AD-2S1 epitope tag; Human Cytomegalovirus; Envelope glycoprotein B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | 8F9 |
| Isotype: | IgG1 |
| Immunogen: | This antibody was generated by isolating B cells from the peripheral blood of a healthy donor who are seropositive for HCMV. |
| Application: | ELISA, FC, IP, WB, NT |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Uniprot ID
P06473
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody binds a 10aa linear epitope AD-2S1 within the extracellular domain of the human cytomegalovirus glycoprotein B and neutralizes the HCMV infection. Reactivity of this antibody to AD-2S1 was confirmed by ELISA. The ability of the antibody to block the infection of human foreskin fibroblasts by HCMV strain AD169 was done by serially diluting the the antibody starting at 15 µg/ml to determine the concentration required for 50% inhibition of infectivity (IC50).The IC50 for the 8F9 IgG1 was ~0.8 µg/ml. It was further confirmed that only the unmutated, primary Ig gH(M):gL(J1) that included the junctional motif seen in 8F9 exhibited detectable binding to gB (PMID:15814702). Both N-terminal and C-terminal AD-2S1 epitope-tagged human GRB2 was detected by 8F9 in a western blot assay. 8F9 was capable of precipitating 80% of the N-terminal and C-terminal AD-2S1 tagged GRB2 from the cell lysates after immunoprecipitation. Immunofluorescence staining was used to detect cells expressing the AD-2S1 tagged GRB2 (PMID:16919678).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
AB02002-10.0-BT Datasheet
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