Anti-Polyglutamine [MW1]

Catalogue Number: AB02003-1.1-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	PolyQ; HTT; htt; Huntington disease protein; HD protein; huntingtin; Glutamine repeats; Atrophin-1
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Mouse
Clone:	MW1
Isotype:	IgG1
Immunogen:	This antibody was raised against a 53 amino acid fragment of atrophin-1 containing poly Q (19) and purified from ascites fluid by protein G affinity chromatography.
Application:	WB, IHC

Overview
Datasheets

Additional Text

Gene Name

HTT

Uniprot ID

P42858

Gene ID

3064

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody strongly prefers to bind the expanded polyQ repeat forms of the mutant huntingtin and displayed no detectable binding to normal huntingtin in Western blot assay. These results suggested that the polyQ domain can assume different conformations that are distinquishable by mAbs. Immunohistochemistry with wild type (WT) and mutant Htt transgenic mouse (R6) brains shows that different antibodies have different staining patterns. MW1 shows punctate cytoplasmic and neuropil staining in immunohistochemical staining (PMID: 11719267). When MW1 scFv is co-transfected in 293 cells with htt exon 1 containing an expanded polyQ domain, it stimulate htt aggregation and apoptosis (PMID: 11792860). The crystal structure of polyQ bound to MW1, reveals that polyQ adopts an extended, coil-like structure. Consistent with the linear lattice model, multimeric MW1 Fvs bind more tightly to longer than to shorter polyQ tracts and, compared with monomeric Fv, bind expanded polyQ repeats with higher apparent affinities (PMID: 17450152).

AB02003-1.1 Datasheet

Price

£390.00

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