Anti-Spike Protein (RBD) [CV30]
Catalogue Number: AB02019-49.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 ug |
| Host name: | Ferret |
| Clone: | CV30 |
| Isotype: | IgM |
| Immunogen: | The original antibody was generated by isolating B-cells from the peripheral blood mononuclear cells (PBMCs) of SARS CoV2 infected patients, after the serum neutralizing activity was confirmed. |
| Application: | ELISA, FC, NT |
Additional Text
Gene Name
S
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Gene ID
43740568
Uniprot ID
P0DTC2
Specificity
Receptor Binding Domain; SARS-CoV2; SARS Coronavirus 2; SARS-CoV-2; SARS CoV 2; 2019-nCoV; SARS Coronavirus 2 receptor binding domain; SARS CoV2 RBD; Severe acute respiratory syndrome coronavirus 2; Spike protein; S protein; SARS-CoV 2 S protein; S glycoprotein; E2; Peplomer protein
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody is a potent 2019-nCoV neutralizing antibody and highly recommended for diagnosis and/or treatment of SARS CoV 2 or 2019-nCoV. CV30 binds SARS CoV 2 RBD with a very high affinity and achieves 100% neutralization (IC50=0.03µg/ml). ELISA was used to confirm the binding of SARS CoV 2 positive serum samples to the entire ectodomain and the receptor binding domain of the spike protein. Specific binding of the mAb to SARS CoV 2 to receptor binding domain was confirmed with biolayer interferometry. Phycoerythrin labelled mAb was used to stain 293E cells transfected with wild type SARS CoV 2 and were analysed by flow cytometry (Seydoux et al., 2020).
Short Description
This chimeric ferret antibody was made using the variable domain sequences of the original Human format, for improved compatibility with existing reagents, assays and techniques.
AB02019-49.0-BT Datasheet
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