Anti-IgM [1.14]
Catalogue Number: AB02046-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Monoclonal Secondary Antibody - Unconjugated |
| Alias: | Trout immunoglobulin M; immunoglobulin M |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | 1.14 |
| Isotype: | IgG |
| Immunogen: | This antibody was raised by immunizing female BALB/c mice with purified trout IgM. |
| Application: | ELISA, FC, IF, IP, AC, RIP |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody has been used for affinity chromatography with the protein A (Deluca et al, 1983, pmid:6347695). The antibody was shown to be effective in flow cytometry application (Deluca et al, 1983, pmid:6347695). It was demonstrated that this antibody can be utilized in the immunofluorescence staining of trout head kidney, spleen and thymus cells (Deluca et al, 1983, pmid:6347695). Furthermore, it has been shown that this antibody can be used in the immunoprecepitation of trout IgM (Deluca et al, 1983, pmid:6347695). It is used in western blots as a negative against catfish IgL σ (Edholm et al, 2010, pmid:20149930) and MHC class IIβ (Moulana et al, 2008, pmid:18676026). Finally, 1.14 antibody can be used to detect trout antibody in an ELISA (Costa et al, 2011, pmid: 21641928).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB02046-23.0-BT Datasheet
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