Anti-CD18 [H52]
Catalogue Number: AB02054-10.3-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | ITGB2; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta; Complement receptor C3 subunit beta; β2-subunit of leukocyte integrins |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | H52 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with human splenic adherent cells and later boosted with the same type of cells. |
| Application: | FC, IP, Blk, Inh |
Additional Text
Gene Name
ITGB2
Uniprot ID
P05107
Gene ID
3689
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody is successfully capable of inhibiting T cell, natural killer cells and complement receptor type 3 functions. Binding specificity of this antibody was confirmed with immunoprecipitation (PMID: 2580022). H52 mAb blocked 8E5-PHA-blast fusion in a dose-dependent manner with complete inhibition observed at concentrations above 3 µg/ml. Cell fusion (syncytium formation) is a major cytopathic effect of infection by human immunodeficiency virus (HIV). To determine the effect of the mAb on syncytium formation the LFA-1 expression of 8E5 cells and PHA-blasts was checked by flow cytometry. This mAb inhibited HIV-induced syncytium formation, however it did not block binding of gp120 to CD4 (PMID: 2543075). This mAb cross reacts with procine LFA-1 and profoundly inhibited mitogenic and allogenic stimulation of porcine T lymphocytes as well as cytotoxic T cell and natural killer (NK) cell function (PMID: 2481234). The expression of H52 epitope on beta-2 subunit isdependent on its interation with different alpha subunits of the leukocyte integrin LFA-1, Mac-1 and p150,95 and presence of calcium ions. The expression of the H52 epitope was examined by immunoprecipitation (PMID: 9808198).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
AB02054-10.3-BT Datasheet
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