Anti-Spike protein [EY6A]
Catalogue Number: AB02057-15.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 ug |
| Host name: | Human |
| Clone: | EY6A |
| Isotype: | IgM |
| Immunogen: | The original antibody was isolated by cloning antibody genes from blood derived plasmablasts of a Covid-19 infected patient in the convalescent phase. |
| Application: | ELISA, IF, NT, SPR |
Additional Text
Gene Name
S
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Gene ID
43740568
Uniprot ID
P0DTC2
Specificity
SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
EY6A binds S1 domain of SARS-CoV-2 and also cross reacts with SARS-CoV-1. The binding was confirmed by ELISA. Further confirmation of binding of EY6A to SARS-CoV-2 infected cells was done by immunofluorescence. Surface plasmon resonance (SPR) measurements for EY6A Fab showed high affinity binding to immobilised SARS-CoV-2 RBD (KD = 2 nM). EY6A did not completely block binding of ACE2 to RBD, but was successful in neutralizing SARS-CoV-2 infection in Vero E6 cells (Zhou et al., 2020).
Short Description
This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB02057-15.0-BT Datasheet
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