Anti-Spike protein [MR3]

Catalogue Number: AB02059-1.159-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	500 ug
Host name:	Mouse
Clone:	MR3
Isotype:	IgG1
Immunogen:	The original antibody was selected after one round of ribosome display using three high diversity libraries (concave, loop and covex) followed by three rounds of phage display and panning against biotinylated RBD as a bait and increasing the stringency of selection with every round. The last round of selection was against 5nM RBD.
Application:	ELISA, FACS, FC, NT, BLI

Overview
Datasheets

Additional Text

Gene Name

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Gene ID

43740568

Uniprot ID

P0DTC2

Specificity

SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

MR3 is a potent antibody that binds the RBD with an affiinty of KD=1.0 nM and neutralizes SARS-CoV-2 pseudovirus with an IC50 of 0.40 µg m/L. Consistent with its highest affinity, MR3 showed the slowest off-rate (2.3 x 10-4 per second). ELISA was used to screen the libraries to identify specific RBD binders. Neutralization activity of the sybody was checked by pre-incubating pseudoviral particles with different concentrations of sybody before infection of VeroE6-hACE2 cells. The rate of infection was then measured by fluorescence-activated cell sorting (FACS). The binding kinetics between sybodies and the RBD was assessed using bio-layer interferometry (Li et al., 2020).

Short Description

This chimeric mouse antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

AB02059-1.159-BT Datasheet

Price

£2,190.00

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Quantity
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