Anti-Glycoprotein B of HSV [Fd79]
Catalogue Number: AB02069-15.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | gB; HSV gB; Herpes simplex virus; Envelope glycoprotein B; UL27 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | Fd79 |
| Isotype: | IgM |
| Immunogen: | This antibody was raised against Herpes simplex virus. |
| Application: | FC, IF, NT |
Additional Text
Purification
Purified
Uniprot ID
P10211
Antibody Clonality
Recombinant Monoclonal
Gene ID
24271469
Gene Name
UL27
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody protected mice against encephalitis and death following ocular inoculation with the virus. Fd79 (anti-gB) prevented encephalitis even when immunization was delayed until 48 hours post-infection. It significantly reduced the severity of epithelial keratitis and lowered the frequency of persistent viral infection in an outbred mouse model. Murine Fd79 has been shown to neutralize HSV-1 in vitro without complement. Both humanized and murine Fd79 cause a 90% reduction of plaques at an antibody concentration of 7 nM (1 ug/ml) (PMID: 3010559, 3030642, 2838428). The binding of antibody to HSV-infected Vero cells was tested using fluorocytometry. Immunostaining was used to view Vero cell monolayers infected with HSV-1 and total cell lysis in the presence of Fd79. Humanized version of this antibody saw a two fold decrease in affinity (PMID: 1849279).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB02069-15.0 Datasheet
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