Anti-CD32 [MDE-8]
Catalogue Number: AB02072-10.3-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; FCGR2A; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | MDE-8 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund's adjuvant. |
| Application: | ELISA, FC, Blk |
Additional Text
Gene Name
FCGR2A
Uniprot ID
P12318
Gene ID
2212
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding ability of MDE-8 to FcgammaRIIa was confirmed using flow cytometry. MDE-8 binds to FcgammaRIIa on cell lines, peripheral bloodmonocytes, PMN, platelets and blocked ligand binding via this receptor. It can also effectively blocked FcγRIIa ligand-binding via its F(ab')2 fragment. Overnight incubation of monocytes with F(ab')2 fragments of MDE-8 leads to a 60% decrease in cell surface expression of FcγRIIa. MDE-8 whole antibody induced a concomitant 30% decrease of FcγRI on THP-1cells and monocytes (PMID: 15982355). MDE-8 was reoported to induce Fc-mediated hypothermia and severe thrombocytopenia in FCGR2A mice (PMID: 26396093).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB02072-10.3-BT Datasheet
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