Anti-CD32 [MDE-9]
Catalogue Number: AB02073-10.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; FCGR2A; FcgammaRIIa-H131; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Human |
| Clone: | MDE-9 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund's adjuvant. |
| Application: | ELISA, FC, IF, IHC, Blk |
Additional Text
Gene Name
FCGR2A
Uniprot ID
P12318
Gene ID
2212
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
MDE-9 was able to detect FcgammaRIIa-H131 in both flow cytometry and immunohistochemistry. MDE-9 bound FcgRIIa-H131 on cell lines expressing FcgRIIaH131, as well as on peripheral blood monocytic cells and PMN in immunofluorescence assays. MDE-9 was used to develop an FcgammaRIIa allotyping method based on flow cytometry. The results of flow cytometric FcgammaRIIa allotyping correlated completely with PCR genotyping. This novel allotyping assay can facilitate the screening of patients in a routine diagnostic setting. A combination of MDE-9 and mAb 41H16 can be used in FcgammaRIIa-H/H131 homozygous individuals to detect FcgammaRIIa and FcgammaRIIb surface expression on monocytes (PMID: 15604023).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB02073-10.0 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations