Anti-Mesothelin [C10]

Catalogue Number: AB02078-10.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; SS scFv
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Human
Clone:	C10
Isotype:	IgG1
Immunogen:	The original parent antibody SS scFv was generated by intradermally immunizing BALB/c mice with a vector pcD3CAK1-9 which expresses mesothelin under the control of a CMV promoter. Randomized mutations were introduced in the VH and VL CD3 regions to make C10. C10 has S92G-G93S-Y94H mutations in the light chain.
Application:	ELISA, IHC, SPR

Overview
Datasheets

Additional Text

Gene Name

MSLN

Uniprot ID

Q13421

Gene ID

10232

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody is recommended to be used for detection of mesothelin in biological samples. This high affinity anti-mesothelin antibody is derived from its parent antibody SS scFv by incorporating S92G-G93S-Y94H mutations in the light chain. The resulting antibody C10 has a 55 times higher affinity towards mesothelin in an ELISA as compared to its parent antibody. The binding characteristics and affinity of the antibody for mesothelin was determined by surface plasmon resonance (BIAcore assay). C10 binds mesothelin with an affinity of Kd = 0.2 nM. Immunoconjugates with C10 as targeting agent and Pseudomonas exotoxin A (PE38) as immunotoxin can be used for identifying samples containing mesothelin through immunohistochemical assays. The cytotoxic activity of the immunotoxin was also checked on antigen positive A1847 cells (PMID: 10385321, 9435250).

Short Description

This full-length, chimeric human antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.

AB02078-10.0 Datasheet

Price

£385.00

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Quantity
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