Anti-TGEV Coronavirus Spike glycoprotein [1AF10]
Catalogue Number: AB02090-3.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Spike glycoprotein; spike protein; S glycoprotein; E2; Peplomer protein; Porcine transmissible gastroenteritis coronavirus; 1A.F10 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | 1AF10 |
| Isotype: | IgG2b |
| Immunogen: | The original antibody was generated by immunizing mice with native transmissible gastroenteritis virus (TGEV). |
| Application: | ELISA, WB, RIA, NT, Crstion |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Uniprot ID
P07946
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Short Description
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Application Notes
This antibody is capable of neutralizing TGEV and recognizing it in an immunodot blot assay. The antigenic site of the S glycoprotein of TGEV where the monoclonal antibody binds was identified using competitive RIA (Correa et al., 1988; PMID: 2453977). Additionally, this antibody can recognize the TGEV spike protein in ELISA and be used to detect site A of the S protein in Western blot analysis, specifically for investigating the effect of glycosylation on antibody binding (Gebauer et al., 1991; PMID: 1711257). The Fab format of this antibody was crystallized in complex with the TGEV coronavirus Spike fragment (Reguera et al., 2012; PMID: 22876187).
AB02090-3.0-BT Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations