Anti-MUC1 [HMFG1 (1.10.F3)]

Catalogue Number: AB02206-10.3-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Human
Clone:	HMFG1 (1.10.F3)
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing mice with human mammary milk fat globule.
Application:	ELISA, IF, IP, WB, RIA, IHC

Overview
Datasheets

Additional Text

Gene Name

MUC1

Gene ID

4582

Uniprot ID

P15941

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Specificity

CD227; Muc-1; Mucin-1; Milk mucin; human milk fat globule antigen; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA

Application Notes

This antibody reacts with most of the breast cancer cells lines and with epithelial cells of human milk. The antibody also reacts with neoplasms of epithelial origin, particularly carcinomas of breast, ovary, cervix, bronchus and gastrointestinal tract. This antibody was used for the study of expression of epithelial antigen in normal and disordered cervical squamous epithelium using immunohistological methods (PMID: 6315048). This antibody can recognize the epithelial membrane antigen in a radioimmunoassay. Immunofluorescent staining of cultures of human breast cells with these antibodies demonstrated that they are heterogeneous in their distribution (PMID: 6086018). HMFG1 and HMFG2 recognize different epitopes of the millk fat globule (PMID: 6086018, 2424847). This antibody was used for the study of tumor associated antigenic epitopes purified from human skimmed milk using immunoaffinity chromatography, immunoprecipitation and immunoblot techniques (PMID: 2443241). The reshaped humanized antibody, HuHMFGl, showed no difference in relative affinity for its antigen compared with the parent mouse HMFG1 (PMID: 7682986). This mAb reacts with P1-24 synthetic peptide (PDTRPAPGSTAPPAHGVTSAPDTR) representing the repeating amino acid sequence of the mucin core protein and also reacted strongly with ovarian carcinoma-associated sebaceous gland antigen (SGA) by direct binding and in a sandwich ELISA assay with OM-1 as the capture Mab (PMID: 1697426).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB02206-10.3 Datasheet

Price

£390.00

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