Anti-IL-5 [JES1-39D10]

Catalogue Number: AB02207-10.3-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	IL5; Interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Human
Clone:	JES1-39D10
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing a male lewis rat with semi-purified recombinant mammalian-expressed IL-5.
Application:	ELISA, FC, ICC, IHC, Blk

Overview
Datasheets

Additional Text

Gene ID

3567

Gene Name

IL5

Uniprot ID

P05113

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody can effectively block IL-5 induced HL-60 histamine content and TF-1 proliferation. 39D10 was also evaluated for its ability to neutralize the ILJ-dependent growth of eosinophilbasophil colonies (i.e., both histamine-positive and histaminenegative Eo-type) in methylcellulose culture. This antibody did not cross-react with mouse IL-5 in indirect ELISA (PMID: 1706953). This antibody was used in immunohistochemical method to study the localisation and phenotype of individual cytokine producing cells in synovial biopsy specimens in rheumatoid arthritis (PMID:7677442). This antibody was also used in immunocytochemical and a flow cytofluorimetric method to detect intracellular IFN-g, IL-4 and IL-5 in T-cell clones, peripheral blood mononuclear cells PBMC and bronchoalveolar lavage fluid BALF cells (PMID: 9134033). The original rat IgG2a antibody binds human IL-5 with a binding affinity of Kd= 53 pM (PMID: 7613159). This antibody can be used in combination with JES1-5A10 for the detection of cytokines in a capture ELISA (PMID: 16827896). Humanized version of this antibody CMX5-2 and CMX5-5 had lower affinities for IL-5 than 39D10, with Kd values that were 1.4 times the Kd for 39D10 (PMID: 8844835).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

AB02207-10.3-BT Datasheet

Price

£1,655.00

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