Anti-IFNAR-1 [64G12]

Catalogue Number: AB02215-1.1-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	IFNAR1; IFN-R-1; Interferon alpha/beta receptor 1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; CRF2-1; Type I interferon receptor 1
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Mouse
Clone:	64G12
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing mice with recombinant soluble interferon (r sIFN-R) expressed and purified from E.coli or from a culture supernatant of Cos7 cells.
Application:	ELISA, FC, IF, IP, WB, CM, NT, Inh, Mcrspy

Overview
Datasheets

Additional Text

Gene ID

3454

Gene Name

IFNAR1

Uniprot ID

P17181

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody is capable of nuetralizing the biologic activity of IFN-alpha, -beta, and -omega and natural type I IFN, but not IFN-gamma. The receptor-blocking anti-IFNAR-1 mAb 64G12 also inhibited IFN-a2a-induced ISGF3 and IFN regulatory factor-1 (IRF-1) binding to their ISRE. It is also reported that the affinity of this antibody for the cellular receptor depends on the cell line used. The binding characterization of this antibody was done using ELISA and western blotting (PMID: 8423335). The reactivity of the monoclonal antibodies (mAbs) recognizing the recombinant soluble IFN-R was tested against the natural class I receptor expressed at the surface of Daudi cells, by membrane immunofluorescence. Antibody 64G12 both inhibits the binding of radiolabeled interferon-α2 and IFN-α8 to their cell surface receptors and neutralizes the antiviral and antiproliferative actions of all the type I interferons tested, including IFN-β, IFN-ω, and human leukocyte IFN, a mixture of different interferon-α isotypes. Immunoprecipitation followed by surface iodination demonstrated that the neutralizing MAb 64G12 recognizes a protein of 105 kD in extracts of Daudi cells (PMID: 7584665). This antibody inhibits activation of the Jak-Stat signal transduction pathway, administered together with a subeffective dose of cyclosporine induced prolonged survival of skin allografts in major histocompatibility complex (MHC) divergent cynomolgus monkeys (PMID: 9568730). This antibody was also used to study the expression of IFN-α/β receptor expression on a number of different human cell lines including Daudi, K562, Ly28 and HL60 using confocal microscopy and flow cytometry (PMID: 12040445).

AB02215-1.1-BT Datasheet

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£1,655.00

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