Anti-Disialoganglioside 2 [5F11]
Catalogue Number: AB02233-10.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | GD2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Human |
| Clone: | 5F11 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing mice with ganglioside G.sub.D2..sup.9. |
| Application: | ELISA, FC, IF |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody is recommended to be used in the detection of GD2 positive tumor cells in the marrow specimens using immunofluorescence staining (PMID: 15136638). It is also recommended for staining of neuroblastoma cells and analysing them using flow cytometry (PMID: 2526848). The anti-GD2 scFv 5F11 recombinantly fused to streptavidin (5F11-scFv-SA) formed a homo-tetramer and showed high GD2 binding. 5F11-scFv-SA allowed inhibition of the binding of anti-5F11 antiidiotypic antibody 1G8 to 5F11 in an enzyme-linked immunosorbent assay (PMID: 15136638). Diphtheria toxins fused to scFv 5F11 demonstrated specific killing of GD2+ tumor cells independent of host immune function (PMID: 11877684). This antibody was also used for the development of bispecific antibody (5F11-scBA) to target disialoganglioside (GD2) positive tumors (PMID: 27471647).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
AB02233-10.3 Datasheet
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