Anti-HPi2 [HIC1-2B4 ]

Catalogue Number: AB02240-10.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Human Pancreas Endocrine Islet Cell Marker 2; Human pancreatic marker; Endocrine pan-islet cell marker
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Human
Clone:	HIC1-2B4
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing BALB/c mice with endocrine cells of the islets of Langerhans.
Application:	FC, IF, IHC

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody binds distinct surface molecules expressed on endocrine cells and have been validated for a number of experimental methods including immunohistochemistry and live cell sorting by flow cytometry. This antibody can be used individually or in combination with others to isolate populations of α, β, exocrine, or duct cells from primary human pancreas by FACS and to characterize the detailed cell composition of human islet preparations (PMID: 19383399, 27641999). This antibody was used in combination with HIC3-2D12 to identify and isolate major subtypes of the human islet cells including α- and β-cells and the purity levels of the isolated cells was determined using immunofluorescence staining (PMID: 31780397). Humanized version of this antibody has also been reported (PMID: 29758224). Chimeric antigen receptor (CAR) with scFv form of this antibody was used in the novel Tregs-based antigen-specific Type 1 Diabetes (T1D) immunotherapy (PMID: 33068914).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB02240-10.0 Datasheet

Price

£390.00

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