Anti-CD123 [7G3]
Catalogue Number: AB02242-2.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | IL3RA; IL-3RA; IL-3R-alpha; Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | 7G3 |
| Isotype: | IgG2a |
| Immunogen: | The original antibody was generated by intraperitoneally immunizing BALB/c mice with 5 X lo6 COS cells transfected with the IL-3R a-chain in combination with adjuvant peptides. |
| Application: | FC, IP, WB, Blk |
Additional Text
Gene Name
IL3RA
Gene ID
3563
Uniprot ID
P26951
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antagonist antibody binds IL-3Rα and blocks IL-3 binding and IL-3-mediated functions. mAb 7G3 immunoprecipitated and recognized in western blots the IL-3R α chain expressed by transfected cells and bound to primary cells expressing IL-3Rα. The expression of IL-3Rα chain on various cells types was analysed by flow cytometry using this antibody (PMID: 8547680). It is reported that 7G3 inhibits IL-3Rα and βc dimerization (PMID: 8649415). This antibody was used in the study of neutrophils expressing IL-3 receptors which are activated by granulocyte-macrophage colony-stimulating factor or GM-CSF (PMID: 7579364). The original 7G3, mouse IgG2a antibody inhibited IL-3-mediated intracellular signaling of isolated acute myeloid leukemia (AML) CD34+ CD38- cells in vitro and reduced their survival (PMID: 19570512). Humanized version of this antibody CSL362 is available on request. CSL362, retains the ability to neutralise IL-3 and has enhanced affinity for the FcγRIIIa (CD16) on NK cells. In vitro studies have demonstrated that the increased affinity for CD16 correlates with greater antibody-dependent cell-mediated cytotoxicity (ADCC) against CD123 expressing cell lines (Busfield et al., 2012). CSL362 is being accessed for use in the treatment of myeloid leukemia (PMID: 24363400, 2524888, 26130514).
AB02242-2.0 Datasheet
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