Anti-PD-1 [JC053]
Catalogue Number: AB02247-202.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD279; Programmed death 1 receptor; PD1; PD 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Dog |
| Clone: | JC053 |
| Isotype: | IgG4 |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with recombinant canine PD-1 using an exponentially increasing dosing strategy with MPLA as adjuvant. |
| Application: | ELISA, FC, WB, IHC |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Uniprot ID
A0A024FCJ9
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody is recommended for detection of canine PD-1. This antibody can detect canine PD-1 in a western blot assay. JC053 successfully detected canine PD-1 expressed on formalin-fixed paraffin-embedded (FFPE) tonsil and lymph node via immunohistochemistry suggesting that this could be useful for histological detection of canine PD-1 and as diagnostic tool in tumor biopsies. JC053 was also used to determine the PD-1 expression on CD4+ and CD8+ T-cells of canine PBMCs stimulated with ConA using flow cytometry. It could also detect recombinant canine PD-1 expression on CHO and CLGL-90 cells in flow cytometry. The ability of the antibody to block interactions between PD-1 and PD-L1 was checked using ELISA. It is known that blockade of PD-L1 and PD-1 interactions causes increase in Ifn-γ level. This antibody did not effectively block PD-1 and did not cause a recovery of T cell function (PMID: 32614928).
Short Description
This chimeric dog antibody was made using the variable domain sequences of the original Mouse IgA format, for improved compatibility with existing reagents, assays and techniques.
AB02247-202.1-BT Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations