Anti-Cavbeta1 Ca2+ channel [N7/18.1]

Catalogue Number: AB02250-10.3-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:N7/18.1R; Voltage-dependent L-type calcium channel subunit beta-1; CAB1; Calcium channel voltage-dependent subunit beta 1
Shipping Condition:Blue Ice
Unit(s): 200 ug
Host name: Human
Clone: N7/18.1
Isotype: IgG1
Immunogen: This antibody was raised by immunising BALB/c mice with a synthetic peptide of amino acids 19-34 of rat Cav1.
Application: IF, IP, WB

Additional Text

Gene Name

CACNB1

Uniprot ID

P54283

Gene ID

50688

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The IgG2a version of this antibody was used for immunofluorescence on primary myoblasts from 1-null muscles (5, 9) infected with 1a cDNA-containing virions at a multiplicity of infection of 0.5 (Eltit et al, 2014; PMID:25384984). Immunoprecipitation has been preformed on HEK293 cell lysates using the IgG2a version of this antibody (Morgenstern et al, 2019; PMID:31403402). This antibody has been used for immunofluorescence on mouse hippocampal cultures (Held et al, 2020; PMID:32616470). Immunoprecipitation was preformed with this antibody on the lysate of mouse hemibrains ( Voskobiynyk, 2020; PMID:32657270). This antibody was used in immunoblotting of myoblasts stably transduced with Wt-β1a or β1a-V156A subunit (Perez, 2018; PMID:29212769). Immunofluorescence was preformed on mouse diaphraghms by using this antibody (Kaplan et al, 2018; PMID:29949772).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.