Anti-GFP [N86/38.1]
Catalogue Number: AB02257-10.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | N86/38.1R; Green fluorescent protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | N86/38.1 |
| Isotype: | IgG1 |
| Immunogen: | This antibody was raised by immunising BALB/c mice with a fusion of protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein. |
| Application: | IF, IP, IHC |
Additional Text
Uniprot ID
P42212
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
Immunofluorescence was preformed with this antibody in the brain of mice. Here it was bound to both yellow and green fluorescent protein (Kumar et al, 2015; PMID:25337655). This antibody was used for immunoprecipitation on Elav-GAL4, 50Y-GAL4, UAS-eGFP, The UAS-GFP and RpL10A fly strains of the genus Drosophila (Thomas et al, 2012; PMID:22792260). Western blotting was preformed with this antibody on samples of Elav-GAL4, 50Y-GAL4, UAS-eGFP, The UAS-GFP and RpL10A fly strains of the genus Drosophila (Thomas et al, 2012; PMID:22792260). Immunohistochemistry was preformed on zebrafish to visualize GFP (Mackenzie and Raible, 2012; PMID:23094043). Immunoprecipitation using this antibody was preformed on the sciatic nerves of rats infected with an adenovirus containing a GFP construct (Chang et al, 2014; PMID:25362471).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
AB02257-10.0-BT Datasheet
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