Anti-GABA(B)R [N93A/49.2]

Catalogue Number: AB02260-10.3-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:N93A/49.2R; Gamma-aminobutyric acid type B receptor subunit 1; Gabbr1; GABA-B receptor 1; GABA-B-R1; GABA-BR1; GABABR1; Gb1
Shipping Condition:Blue Ice
Unit(s): 200 ug
Host name: Human
Clone: N93A/49.2
Isotype: IgG1
Immunogen: This antibody was raised by immunising BALB/c mice with a fusion protein of amino acids 873-977 (cytoplasmic C-terminus) of rat GABABR1.
Application: IF, IP, PLA

Additional Text

Gene Name

GABBR1

Gene ID

81657

Uniprot ID

Q9Z0U4

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody was used in a proximity ligation assay on HEK293 cells transiently transfected using Lipofectamine 2000 with cDNA plasmids expressing GABABR1-eYFP, GABABR2 and GIRK2a (Li et al, 2020; PMID:32111696). Immunofluorescence was preformed on the hippocampal slices of P7 male Wistar rats using this antibody (Wright et al, 2017; PMID:28450542). An immunoprecipitation was preformed with this antibody on lysates from hippocampal slices of P7 male Wistar rats (Wright et al, 2017; PMID:28450542). Immunofluorescence was preformed on the brain of Tg(Gng3-GFP)- HK208Gsat and KI(Gng7-3-IRES-GFP) mice (Schwindinger et al, 2012; PMID:22207761).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.