Anti-Covid-19 & SARS-CoV Nucleocapsid [1C7C7]
Catalogue Number: AB02325-49.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | nucleoprotein; NP; NC; Protein N; Nucleocapsid protein; SARS-CoV Protein N; SARS-CoV-2 Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 1C7; severe acute respiratory syndrome virus |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Ferret |
| Clone: | 1C7C7 |
| Isotype: | IgM |
| Immunogen: | The original version of this antibody was raised against SARS-CoV-1/2. |
| Application: | ELISA, FC, WB, IHC |
Additional Text
Gene Name
N
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Gene ID
43740575
Uniprot ID
P0DTC9
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The antibody 1C7C7 detects SARS-CoV-2 and SARS-CoV-1 and can be used in affinity binding assays, flow cytometry, immunocytochemistry, and western blotting. Its effectiveness in detecting the nucleocapsid of the novel coronavirus causing COVID-19 was confirmed in multiple studies. For instance, it was used to analyze SARS-CoV-2 nucleoprotein extracted from infected cells via Western blot (Hoagland et al., 2020) ,and similarly by Blanco-Melo et al. in a study on imbalanced host response to SARS-CoV-2 (2020; pmid: 32416070). Another group has used this clone to stain infected cells fixed with 10% neutral formalin during a neutralization assay (Piepenbrink et al., 2020). Daniloski et al. performed similar staining procedure with 1C7C7 for a trans-complementation assay (2020; pmid: 33147445). Finally, this antibody was also used to stain SARs-CoV-2 infected cells in a study on in vitro susceptibility to SARS-CoV-2 infection (Dobrindt et al., 2020; pmid: 32995783).
Short Description
This chimeric ferret antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
AB02325-49.0 Datasheet
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