Anti-Ft OAg [Ab52]
Catalogue Number: AB02346-201.4-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | O-antigen of Francisella tularensis; O-antigen ligase family protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Dog |
| Clone: | Ab52 |
| Isotype: | IgG2 |
| Immunogen: | The original antibody was generated by repeatedly immunizing BALB/c mice with live vaccine strain (LVS) and F. tularensis lipopolysaccharide admixed with CpG ODN 1826 (TCC ATG ACG TTC CTG ACG TT) (an oligodeoxynucleotide containing unmethylated CpG dinucleotides with fully phosphorothioate backbone. |
| Application: | ELISA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Uniprot ID
A0A0B6E216
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This potent binder can be used to detect the O antigen of F. tularensis. The antigen specificity of this antibody was tested using ELISA. It is capable of binding to heat killed live vaccine strain and SchuS4 F. tularensis strains in an ELISA. This antibody was also capable of detecting the longer chains of the lipopolysaccharide ladder in a western blot assay (PMID: 21466282). Ab52 can be used as a reporter to monitor the response of vaccine recipients to protective B-cell epitopes of F. tularensis OAg (PMID: 22486311).
Short Description
This chimeric dog antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
AB02346-201.4-BT Datasheet
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