Anti-CD19 [MB19-1]

Catalogue Number: AB02364-201.1-BT-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:B-lymphocyte antigen CD19; Differentiation antigen CD19; B-lymphocyte surface antigen B4; B4; CD19 molecule; CD19 antigen
Shipping Condition:Blue Ice
Unit(s): 1 mg
Host name: Dog
Clone: MB19-1
Isotype: IgG2
Immunogen: This antibody was raised by immunising CD19-deficient mice with a cell line of mCD19+ mouse pre-B cells.
Application: ELISA, FC, IF

Additional Text

Gene Name

CD19

Gene ID

12478

Purification

Purified

Uniprot ID

P25918

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This clone can be used to detect and analyze the presence of the CD19 transmembrane protein. The specificity towards the protein has been confirmed using ELISA and immunofluorescence (Sato et al., 1996; PMID: 8906812). Additionally, the MB19-1 antibody has been used in several flow cytometry assays. One group used this antibody to determine the differences in CD19 distribution between cells of young and old mice (Yamamoto et al., 2018; PMID: 29625072). Another group used it to differentiate eosinophils from other leukocytes (Vanella et al., 2017; PMID: 27043413). A final group used flow cytometry to determine the fraction of B cells cells in a sample with this antibody (Bedaret et al., 2009; PMID: 19907079).

Short Description

This chimeric dog antibody was made using the variable domain sequences of the original Mouse IgA format, for improved compatibility with existing reagents, assays and techniques.