Anti-lambda light chain [4-D11]
Catalogue Number: AB02370-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Monoclonal Secondary Antibody - Unconjugated |
| Alias: | B cell IgL; Ig; ferret immunoglobulin; lambda light chain; D11 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | 4-D11 |
| Isotype: | IgG1 |
| Immunogen: | The original mouse IgG1 version of this antibody was raised against ferret immunoglobulins (IgG + IgM). |
| Application: | ELISA, FC, WB |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody is specific for the ferret kappa light chain of B cell surface receptor (BCR) and enables segreation of kappa and lambda B cells representing great improvement over polyclonal anti-ferret Ig. It can be used as an indicator of B cell malignancies such as multiple myeloma. It was screened for reactivity against ferret peripheral blood mononuclear cells by FCM what demonstrated specificity for CD79B+ B cells (Kirchenbaum and Ross, 2017; pmid:28286781). It is suitable for Western Blot, ELISA and Flow Cytometry applications. Enzyme-linked immunosorbent assay with the murine version of this antibody (ELISA) was used to assess the reactivity against purified ferret Ig and to determine the IgG subclass and concentration of the respective mAb (Kirchenbaum and Ross, 2017; pmid:28286781). Further, a competitive ELISA was performed using unlabeled and biotinylated MαF-Ig mAb to identify overlapping epitope recognition (Kirchenbaum and Ross, 2017; pmid:28286781). To characterize the specificity of the murine version of this antibody a western blot on ferret immunoglobin was preformed (Kirchenbaum and Ross, 2017; pmid:28286781).
AB02370-1.1-BT Datasheet
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