Anti-SARS-CoV-2 and SARS-CoV nucleocapsid [mBG17]

Catalogue Number: AB02382-48.1-BT-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:NP; nucleoprotein; NC; Protein N; Nucleocapsid protein; SARS-CoV-2 Protein N; SARS-CoV-2 Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 17; SARS-CoV nucleocapsid
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 1 mg
Host name: Ferret
Clone: mBG17
Isotype: IgG1
Immunogen: The original mouse IgG1 version of this antibody was raised by immunizing BALB/c mice with a recombinant SARS-Cov-2 N protein corresponding to AA133-419.
Application: ELISA, IF, WB

Additional Text

Gene Name

N

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Gene ID

43740575

Uniprot ID

P0DTC9

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 and SARS-Cov was confirmed by Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 and SARS-Cov was confirmed by ELISA (Terry et al., 2021; pmid: 33714753). This antibody detected SARS-Cov-2 N protein in Western blot exhibiting labeling patterns suggesting a linear epitope (Terry et al., 2021; pmid: 33714753). mBG17 was shown to be very effective in staining SARS-Cov-2 N protein in immunofluorescence in SARS-Cov-2-infected Vero cells, both methanol and paraformaldehyde fixed (Terry et al., 2021; pmid: 33714753). Finally, the original version of mBG17 was demonstrated to be an effective detection antibody in a sandwich ELISA assay suggesting it's potential use in SARS-Cov-2 diagnostics (Terry et al., 2021; pmid: 33714753).

Short Description

This chimeric ferret antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.