Anti-1-pHis [SC1-1]
Catalogue Number: AB02384-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | 1-phosphohistidine; N1-Phosphohistidine; histidine |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | SC1-1 |
| Isotype: | IgG |
| Immunogen: | The original rabbit version of this antibody was raised by immunizing rabbits with a 1-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH). |
| Application: | IF, IP, WB, FA, AC |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody can be used for the identification of 1-pHis substrates and functional study of pHis. It was used to assess pHis levels in lysates of various cell lines, such as HPDE6 and pancreatic cancer, FLAG-NME1 293, HeLa, C2C12, and NME1- and NME2-overexpressing (OE) melanoma cell lines via Western blot (Fuhs et al, 2015; pmid: 26140597). A dot blot was also preformed on rabbit antisera, the 1-pTza, 3-pTza peptide libraries, His control library and a pTyr peptide with this antibody demonstrating the antibody's specificity to 1-pHis (Fuhs et al, 2015; pmid:26140597). Phosphorylation of H118 on NDPK-B was assessed as well by immunoblotting with this antibody (Panda et al, 2016; pmid:27453048). SC1-1 was further used to identify the intracellular location of pHis in HeLa cells via immunofluorescence. This clone was shown to be useful for studies on the acidic acidic compartments (i.e., phagosomes, autophagosomes, and lysosomes) since staining is absent from the interior of these acidic compartments and often very bright in the surrounding regions (Fuhs et al, 2015; pmid:26140597). Another group detected 1-pHis in SK-N-SH cells by immunofluorescence staining with this antibody (Adam et al, 2020; pmid:32392889). Furthermore, immunoaffinity purification (IAP) columns, containing protein A crosslinked with this antibody. The samples used were SK-N-BE(2) xenograft tumors from mice (Adam et al, 2020; pmid:32392889). Finally, this clone was also utilized to immunoprecipitate proteins extracted from 10 x 106 SK-N-BE(2) and SK-N-AS neuroblastoma cells (Adam et al, 2020; pmid:32392889).
AB02384-23.0-BT Datasheet
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