Anti-1-pHis [SC50-3]
Catalogue Number: AB02385-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | 1-phosphohistidine; N1-Phosphohistidine; histidine |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | SC50-3 |
| Isotype: | IgG |
| Immunogen: | The original rabbit version of this antibody was raised by immunizing rabbits with a 1-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH). |
| Application: | ELISA, WB, AC |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody can be used for the identification of 1-pHis substrates and functional study of pHis. A dot blot was preformed on rabbit antisera, the 1-pTza, 3-pTza peptide libraries, His control library and a pTyr peptide with this antibody demonstrating its specificity to 1-pHis (Fuhs et al, 2015; pmid:26140597). This antibody has been also used for a western blot on His6-NDPK-A protein (Felix et al, 2020; pmid:32384736). Immunofluorescence was preformed on mouse tissues with this antibody (Yang et al, 2019; pmid:31721359). An ELISA was preformed on 3-day differentiated Sca1+-VPCs with this antibody (Yang et al, 2019; pmid:31721359). Ca1+-VPCs were lysed and immunoprecipitated with this clone (Yang et al, 2019; pmid:31721359). Finally, immunoaffinity purification (IAP) columns, containing protein A crosslinked with this antibody. The samples were SK-N-BE(2) xenograft tumors from mice (Adam et al, 2020; pmid:32392889).
AB02385-23.0-BT Datasheet
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