Anti-3-pHis [SC56-2]
Catalogue Number: AB02386-1.29-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | 3-phosphohistidine; N3-Phosphohistidine; histidine |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | SC56-2 |
| Isotype: | |
| Immunogen: | The original rabbit version of this antibody was raised by immunizing rabbits with a 3-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH). |
| Application: | ELISA, IF, WB, AC |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody can be used for the identification of 3-pHis substrates and functional study of pHis. It was used in an immunoblot performed on four recombinant HK proteins-EnvZ, WalK, ArcB, and EvgS (Kinoshita-Kikuta et al, 2020; pmid:32360198). SC56-2 was also used in Western blot analyses on NDPK-B and PGAM5 proteins (Panda et al, 2016; pmid:27453048) and on mouse liver tissue as well as LX-2 cells (Gong et al, 2020; pmid:32860079). Furthermore, immunoaffinity purification was preformed using this antibody on SK-N-BE(2) xenograft tumors from mice (Adam et al, 2020; pmid:32392889). This clone was also utilized in the immunofluorescence staining (Yang et al, 2019; pmid:31721359). Finally, an ELISA was preformed on 3-day differentiated Sca1+-VPCs with this antibody (Yang et al, 2019; pmid:31721359).
Short Description
This chimeric Fab mouse fragment was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.
AB02386-1.29 Datasheet
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