Catalogue Number: AB02387-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | 3-phosphohistidine; N3-Phosphohistidine; histidine |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | SC39-6 |
| Isotype: | IgG1 |
| Immunogen: | The original rabbit version of this antibody was raised by immunizing rabbits with a 3-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH). |
| Application: | WB, IAP |
Purified
Recombinant Monoclonal
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
This antibody can be used for the identification of 3-pHis substrates and functional study of pHis. SC39-6 can be used to detect 3-pHis via Western blot (Fuhs et al, 2015; pmid: 26140597; Hunter et al., 2019). This clone was also used for phosphoenrichment using immunoaffinity purification (Hunter et al., 2019).
This chimeric mouse antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.