Anti-LDL receptor [12G4]
Catalogue Number: AB02397-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | LDLR; Low density lipoprotein receptor |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | 12G4 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing mice with a peptide corresponding to amino acid residues 195-222 (CDGGPDCKDKSDEENCAVATCRPDEFQC) of the human LDL receptor. |
| Application: | ELISA, FC, IHC, Blk |
Additional Text
Gene Name
LDLR
Gene ID
3949
Uniprot ID
P01130
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding specificity of this antibody was detected using ELISA. This antibody is reported to be used to identify LDL-R expression level in cancer cell lines like HepG2, HeLa, MCF-7, Jurkat, Ramos, HuH7, A549, MDA-MB-231 and Hek293 using flow cytometry. It was found that HepG2 and HeLa cells express LDL-R most strongly. This antibody is also reported to cross react with mouse, rat and hamster LDL receptors. It strongly cross reacts with C2C12, CHO-K1, and YB2/0 cells. 12G4 antibody binding site is the same as that of LDLs and it competes with LDLs for binding site on A549 cells. This antibody was also used for the immunohistochemical analysis of LDL-receptors on cells from the colon, testes, pancreas, gastric mucosa, the basal cell layer of the buccal and exocervical mucosa, and the various renal tubules. It was also used for immunohistochemical analysis of mammary adenocarcinomas (US20090175789).
AB02397-1.1-BT Datasheet
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