Anti-Alpha Actinin 4 [Ab01AA4]
Catalogue Number: AB02447-10.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Non-muscle alpha-actinin 4 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | Ab01AA4 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was raised by immunizing mice with human breast cancer cell line SKBR3. |
| Application: | ELISA, IP, IHC |
Additional Text
Gene Name
ACTN4
Uniprot ID
O43707
Gene ID
81
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
A protein obtained using this antibody is digested with trypsin and analyzed by MALDI-MS. From the analysis it was determined that this protein was alpha-actinin-4 (US9040018). An 8% Tris-glycine gel shows recombinant alpha-actinin-4 protein, and soluble alpha-actinin-4 protein from KATO-3 conditioned cell medium isolated with this antibody (US9040018). ELISA was performed using this antibody on plasma samples (Control: plasma from healthy people, Breast Cancer: plasma from breast cancer patients) from control and patient groups (US9040018). Immunohistochemistry is performed with this antibody using a Actinin-4 IHC Kit on a total of 714 breast tumor and control samples (US9040018).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2 format, for improved compatibility with existing reagents, assays and techniques.
AB02447-10.0-BT Datasheet
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